Journal: Experimental and Therapeutic Medicine

Article Title: Innovative therapeutic strategies for intrauterine adhesions: Role of umbilical cord mesenchymal stem cells in rat models

doi: 10.3892/etm.2025.12805

Figure Lengend Snippet: Experimental grouping for UCMSC administration.

Article Snippet: To track the cellular integration of UCMSC post-transplantation, CellTrackerTM CM-DiI dye (cat. no. C7000; Life Technologies; Thermo Fisher Scientific, Inc.), a lipophilic fluorescent dye, was used for staining the cell membranes.

Techniques: Injection, Biomarker Discovery

Journal: Experimental and Therapeutic Medicine

Article Title: Innovative therapeutic strategies for intrauterine adhesions: Role of umbilical cord mesenchymal stem cells in rat models

doi: 10.3892/etm.2025.12805

Figure Lengend Snippet: Isolation and characterization of UCMSCs. (A) Representative images of the culture process, showing the progression from explanted tissue pieces to 80-90% confluence of spindle-shaped cells with a characteristic ‘whirlpool-like' arrangement. From left to right: Tissue-culture plate, initial tissue adhesion, early cell migration and near-confluent culture. (B) Flow cytometry histograms, demonstrating the expression of mesenchymal stem cell markers (CD90, CD44, CD105, CD73 and CD29) and minimal expression of hematopoietic markers (HLA-DR and CD45). Each panel shows the percentage of cells expressing the respective marker, confirming the mesenchymal phenotype. (C) H&E-stained micrograph of UCMSCs, highlighting elongated, spindle-shaped cells with well-defined, oval nuclei and light red cytoplasm. (D) Fluorescence microscopy images of UCMSCs stained with DAPI (blue) for nuclei, CM-Dil (red) for cell membranes and a merged image showing the co-localization of DAPI and CM-Dil, illustrating cell morphology and integrity. The scale bar represents 50 µm. The data shown are representative of three independent experiments (each data analysis included at least three independent molecular experiments, with a minimum of three animal samples per group in each experiment). UCMSCs, human umbilical cord-derived mesenchymal stem cells.

Article Snippet: To track the cellular integration of UCMSC post-transplantation, CellTrackerTM CM-DiI dye (cat. no. C7000; Life Technologies; Thermo Fisher Scientific, Inc.), a lipophilic fluorescent dye, was used for staining the cell membranes.

Techniques: Isolation, Migration, Flow Cytometry, Expressing, Marker, Staining, Fluorescence, Microscopy, Derivative Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Innovative therapeutic strategies for intrauterine adhesions: Role of umbilical cord mesenchymal stem cells in rat models

doi: 10.3892/etm.2025.12805

Figure Lengend Snippet: Localization and dynamics of human umbilical cord-derived MSCs in rat uterine tissues following transplantation. D3: Early engraftment of fluorescently labeled MSCs (red) with nuclear staining (blue) in all treatment groups. D7: Diverse patterns of cell persistence: Widespread in the IP group, minimal in the IS group and localized at the endometrial-myometrial junction in the IOCV group. D14: Enhanced accumulation near the inner lining of the uterine cavity, suggesting potential integration into the endometrial layer. White arrows indicate initial cell localization. Scale bars, 50 µm. Data are representative of three independent experiments, with three rats per group. D, day; MSCs, mesenchymal stem cells; IP, intraperitoneal; IS, intrauterine site injection; IOCV, intravenous ovary-cervix.

Article Snippet: To track the cellular integration of UCMSC post-transplantation, CellTrackerTM CM-DiI dye (cat. no. C7000; Life Technologies; Thermo Fisher Scientific, Inc.), a lipophilic fluorescent dye, was used for staining the cell membranes.

Techniques: Derivative Assay, Transplantation Assay, Labeling, Staining, Injection

Journal: Experimental and Therapeutic Medicine

Article Title: Innovative therapeutic strategies for intrauterine adhesions: Role of umbilical cord mesenchymal stem cells in rat models

doi: 10.3892/etm.2025.12805

Figure Lengend Snippet: Histological and immunological evaluation of the effectiveness of human umbilical cord-derived mesenchymal stem cell treatment in a rat model of IUA. (A) H&E-stained sections, displaying endometrial thickness among the different experimental groups, namely the normal, IUA, IP, IS and IOCV groups (arrows indicate the endometrial glands). Scale bars represent 100 and 50 µm for the larger and smaller bars, respectively. (B) Masson's trichrome staining highlighting fibrotic areas in the same groups, illustrating variations in fibrosis. (C) A bar graph comparing endometrial thickness among the groups, showing the significantly thicker endometrium in the IP group compared with the IS and IOCV group. (D) A bar graph of the endometrial gland numbers, indicating a higher gland density in the IP group close to normal levels. (E) The percentages of fibrotic areas quantified are shown, with the IP group showing the least fibrosis compared with the other treated and IUA groups. (F) A line graph of IgG levels over time post-transplantation, with the distinct immunological responses among groups highlighted. The IP group's levels initially surged and then stabilized, whereas the IS and IOCV groups showed gradual increases. IUA, intrauterine adhesion; IP, intraperitoneal; IS, intrauterine site injection; IOCV, intravenous ovary-cervix. Data are presented as the mean ± SD. * P<0.05.

Article Snippet: To track the cellular integration of UCMSC post-transplantation, CellTrackerTM CM-DiI dye (cat. no. C7000; Life Technologies; Thermo Fisher Scientific, Inc.), a lipophilic fluorescent dye, was used for staining the cell membranes.

Techniques: Derivative Assay, Staining, Transplantation Assay, Injection

Journal: Experimental and Therapeutic Medicine

Article Title: Innovative therapeutic strategies for intrauterine adhesions: Role of umbilical cord mesenchymal stem cells in rat models

doi: 10.3892/etm.2025.12805

Figure Lengend Snippet: Evaluation of UCMSC therapy in an IUA rat model using various concentrations of UCMSCs. (A) H&E-stained sections, showing endometrial thickness across the treatment groups, namely the Normal, IUA and three UCMSC-dosage (0.5x10 6 , 1.0x10 6 and 5.0x10 6 ) groups (arrows indicate the glands). Scale bars, 100 and 20 µm. (B) Masson's trichrome staining highlighting differences in the fibrotic area among the same groups. (C) A graph of endometrial thickness, illustrating the significant thickness recovery in the 1.0x10 6 group, which was approaching that of the normal levels. (D) A bar graph depicting the number of glands, with the 5.0x10 6 group showing enhanced glandular recovery compared with the other treatment groups. (E) Percentages of the quantified fibrotic areas are shown, indicating the lowest level of fibrosis in the 1.0x10 6 group, which significantly outperformed the other groups. UCMSCs, human umbilical cord-derived mesenchymal stem cells; IUA, intrauterine adhesion. Data are presented as the mean ± SD. * P<0.05.

Article Snippet: To track the cellular integration of UCMSC post-transplantation, CellTrackerTM CM-DiI dye (cat. no. C7000; Life Technologies; Thermo Fisher Scientific, Inc.), a lipophilic fluorescent dye, was used for staining the cell membranes.

Techniques: Staining, Derivative Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Innovative therapeutic strategies for intrauterine adhesions: Role of umbilical cord mesenchymal stem cells in rat models

doi: 10.3892/etm.2025.12805

Figure Lengend Snippet: Fertility restoration in an IUA rat model using UCMSC therapy. (A) Visual representation of co-housing experiments with the Normal, IP/1.0x10 6 (intraperitoneal injection of 1.0x10 6 cells), IS, IOCV and 5.0x10 6 cells groups is shown. (B) The numbers of offspring in the Normal, IUA model and different administration route (IP, IS and IOCV) groups are shown. The IP route showed the highest offspring count among the various treatment groups, although it remained lower compared with that in the Normal group. (C) Comparison of offspring numbers among the cell dosage groups (0.5x10 6 , 1.0x10 6 and 5.0x10 6 cells), demonstrating that a higher dose does not significantly increase fertility restoration compared with the medium (1.0x10 6 cells) dose, while all were below the Normal group outcomes. The data are shown as the mean ± SD ( * P<0.05). The data shown are representative of five independent experiments in each group. IUA, intrauterine adhesion; IP, intraperitoneal; IS, intrauterine site injection; IOCV, intravenous ovary-cervix.

Article Snippet: To track the cellular integration of UCMSC post-transplantation, CellTrackerTM CM-DiI dye (cat. no. C7000; Life Technologies; Thermo Fisher Scientific, Inc.), a lipophilic fluorescent dye, was used for staining the cell membranes.

Techniques: Injection, Comparison

Journal: Nature Communications

Article Title: Intravascular delivery of an ultraflexible neural electrode array for recordings of cortical spiking activity

doi: 10.1038/s41467-024-53720-5

Figure Lengend Snippet: a Postoperative CT images around the estimated penetration site from an example sheep brain, showing the intact brain tissue from three axial planes. The magnified view corresponds to the region in the blue dashed rectangles. Three successive slices spanning 1 mm were shown for each plane. A: anterior, P: posterior, R: right, L: left, S: superior, I: Inferior. Scale bars, 2 cm and 1 cm in the magnified view. b and c , MRI scans (1.5-T, Flair (i), T1 (horizontal and sagittal views, ii and iii)) revealing brain tissue integrity at 7 ( b ) and 30 ( c ) days post the surgery. The magnified views correspond to the region in the blue dashed rectangles in (ii). Scale bars, 2 cm and 1 cm in the magnified view. d Fluorescent images of tissue sections at 30 days after acute uFINE-I implantation, showing no detectable tissue damage caused by implantation. GFAP marks astrocyte activation, a sign of immune response, and DAPI stains nuclei. Scale bar, 50 µm. e Fluorescent images showing two tissue sections along the implantation trajectory (indicated by the pink plane in the inset) after 30-day chronic uFINE-I implantation. Magnified views showing the electrode footprints corresponding to the region in the orange dashed rectangles in the zoomed-out images. NeuN marks mature neurons. Scale bars, 200 µm and 50 µm. f Hematoxylin and Eosin (HE) staining of penetration scar, showing healing with spindle cell hyperplasia 30 days after penetration. The magnified view corresponds to the region in the blue dashed rectangle. Scale bars, 200 µm and 50 µm. d – f The experiment was repeated three times, showing similar results.

Article Snippet: For chronic uFINE-I implantation experiments, to help locate the site of electrode puncture into the brain, we coated the tip of the piercing device with a fluorescent dye (CellTracker CM-DiI, C7000, Thermo Fisher) before implantation.

Techniques: Activation Assay, Staining